PrimePCR™ lncRNA SYBR® Green Assay:PRKAA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0180426

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: PRKAA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: PRKAA2, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103186
Assay Design:   exonic
Chromosome Location:   chr1:56713943-56714061question
Amplicon Length:   89
Refseq Transcripts Amplified:   NM_006252,XM_017001694,XM_017001693,XM_017001692,XR_001737290
Splice Variants Targeted:   ENST00000610361 ENST00000371244
LNCipedia Splice Variants Targeted:   lnc-PRKAA2-8:1

Gene Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses AMPK is activated and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR) key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKAA2
Gene Name:   protein kinase, AMP-activated, alpha 2 catalytic subunit
Aliases:   AMPK, AMPK2, PRKAA
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162409
LNCipedia:   lnc-PRKAA2-8
Entrez:   5563
Chromosome Mapping:   1p31
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download