PrimePCR™ SYBR® Green Assay: TGFA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053322

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TGFA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TGFA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046061
Assay Design:   exonic
Chromosome Location:   2:70692766-70692855question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000295400 ENST00000419940 ENST00000445399 ENST00000418333 ENST00000450929 ENST00000444975 ENST00000394241

Gene Information

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor which activates a signaling pathway for cell proliferation differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   TGFA
Gene Name:   transforming growth factor, alpha
Aliases:   TFGA
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000163235
Entrez:   7039
Chromosome Mapping:   2p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 36.630000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download