PrimePCR™ SYBR® Green Assay: BDNF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054460

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BDNF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BDNF, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047199
Assay Design:   exonic
Chromosome Location:   11:27677827-27677963question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000439476 ENST00000525528 ENST00000395986 ENST00000533131 ENST00000356660 ENST00000418212 ENST00000533246 ENST00000530861 ENST00000395983 ENST00000438929 ENST00000532997 ENST00000395981 ENST00000395978 ENST00000525950 ENST00000314915 ENST00000395980 ENST00000420794

Gene Information

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   BDNF
Gene Name:   brain-derived neurotrophic factor
Aliases:   MGC34632
RefSeq:   NC_000011.9 NT_009237.18 NG_011794.1
Ensembl:   ENSG00000176697
Entrez:   627
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.290000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download