PrimePCR™ lncRNA SYBR® Green Assay:PAFAH1B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0215211

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: PAFAH1B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: PAFAH1B1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0213775
Assay Design:   exonic
Chromosome Location:   chr17:2681762-2681887question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000574816 ENST00000571495 ENST00000574213 ENST00000574468 ENST00000397193 ENST00000572915 ENST00000397195
LNCipedia Splice Variants Targeted:   lnc-RAP1GAP2-5:1 lnc-RAP1GAP2-5:2

Gene Information

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits the other a single subunit. In addition a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq Apr 2009]

Gene Symbol:   PAFAH1B1
Gene Name:   platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Aliases:   LIS1, LIS2, MDCR, MDS, PAFAH
RefSeq:   NC_000017.10 NG_009799.1 NT_010718.16
Ensembl:   ENSG00000007168
LNCipedia:   lnc-RAP1GAP2-5
Entrez:   5048
Chromosome Mapping:   17p13.3
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download