PrimePCR™ lncRNA SYBR® Green Assay:TOP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0181471

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: TOP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: TOP1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0104231
Assay Design:   intron_spanning
Chromosome Location:   chr20:41061461-41076260question
Amplicon Length:   89
Refseq Transcripts Amplified:   NM_003286
Splice Variants Targeted:   ENST00000361337
LNCipedia Splice Variants Targeted:   lnc-PLCG1-3:1

Gene Information

This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq Jul 2008]

Gene Symbol:   TOP1
Gene Name:   topoisomerase (DNA) I
Aliases:   TOPI
RefSeq:   NC_000020.10 NG_012262.1 NT_011362.10
Ensembl:   ENSG00000198900
LNCipedia:   lnc-PLCG1-3
Entrez:   7150
Chromosome Mapping:   20q12-q13.1
Gene Strand:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download