PrimePCR™ SYBR® Green Assay: BGLAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041159


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PrimePCR™ PreAmp for SYBR® Green Assay: BGLAP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: BGLAP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038437
Assay Design:		exonic
Chromosome Location:		1:156211968-156212066question
Amplicon Length:		69
Splice Variants Targeted:		ENST00000305628 ENST00000317643 ENST00000397558 ENST00000269051 ENST00000536287 ENST00000288071 ENST00000269554 ENST00000483320 ENST00000524821 ENST00000313465 ENST00000555427 ENST00000555147 ENST00000367840 ENST00000432587 ENST00000441788 ENST00000395095 ENST00000313639 ENST00000331565 ENST00000450985 ENST00000598234 ENST00000359827 ENST00000321063 ENST00000354287 ENST00000378617 ENST00000341666 ENST00000451343 ENST00000295367 ENST00000331860 ENST00000307098 ENST00000361069 ENST00000355452 ENST

Gene Information

Description Not Available

Gene Symbol:		BGLAP
Gene Name:		bone gamma-carboxyglutamate (gla) protein
Aliases:		BGP, OC
RefSeq:		NC_000001.10 NT_004487.19
Ensembl:		ENSG00000242252
Entrez:		632
Chromosome Mapping:		1q25-q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	37.060000
Efficiency	92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.