PrimePCR™ SYBR® Green Assay: AP1B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050024


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PrimePCR™ PreAmp for SYBR® Green Assay: AP1B1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: AP1B1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042762
Assay Design:		exonic
Chromosome Location:		22:29745237-29745351question
Amplicon Length:		85
Splice Variants Targeted:		ENST00000405198 ENST00000317368 ENST00000402502 ENST00000421126 ENST00000415447 ENST00000357586 ENST00000356015 ENST00000432560

Gene Information

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large one medium and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:		AP1B1
Gene Name:		adaptor-related protein complex 1, beta 1 subunit
Aliases:		ADTB1, AP105A, BAM22, CLAPB2
RefSeq:		NC_000022.10 NT_011520.12
Ensembl:		ENSG00000100280
Entrez:		162
Chromosome Mapping:		22q12,22q12.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.310000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.