PrimePCR™ SYBR® Green Assay: TSPO, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050031


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PrimePCR™ PreAmp for SYBR® Green Assay: TSPO, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TSPO, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042769
Assay Design:		exonic
Chromosome Location:		22:43558916-43559025question
Amplicon Length:		80
Splice Variants Targeted:		ENST00000337554 ENST00000583777 ENST00000329563 ENST00000396265 ENST00000368008 ENST00000361127 ENST00000376372 ENST00000300209 ENST00000333012 ENST00000367397 ENST00000303334 ENST00000394037 ENST00000543856 ENST00000335350 ENST00000373192 ENST00000602067 ENST00000546292 ENST00000538115 ENST00000542585 ENST00000546213

Gene Information

Present mainly in the mitochondrial compartment of peripheral tissues the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding and the other variants encode the same protein. [provided by RefSeq Feb 2012]

Gene Symbol:		TSPO
Gene Name:		translocator protein (18kDa)
Aliases:		BPBS, BZRP, DBI, IBP, MBR, PBR, PBS, PKBS, PTBR, mDRC, pk18
RefSeq:		NC_000022.10 NT_011520.12
Ensembl:		ENSG00000100300
Entrez:		706
Chromosome Mapping:		22q13.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998100
y-intercept	36.500000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.