PrimePCR™ SYBR® Green Assay: DMPK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029376

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DMPK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DMPK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014430
Assay Design:   Intron-spanning
Chromosome Location:   19:46281778-46282575question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000458663 ENST00000342805 ENST00000291270 ENST00000447742 ENST00000377750 ENST00000343373 ENST00000348168 ENST00000354227

Gene Information

The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin the beta-subunit of the L-type calcium channels and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5000 copies causes myotonic dystrophy type I which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DMPK
Gene Name:   dystrophia myotonica-protein kinase
Aliases:   DM, DM1, DM1PK, DMK, MDPK, MT-PK
RefSeq:   NC_000019.9 NG_012745.1 NT_011109.16 NG_009784.1
Ensembl:   ENSG00000104936
Entrez:   1760
Chromosome Mapping:   19q13.3

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