PrimePCR™ SYBR® Green Assay: VAMP7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030537


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PrimePCR™ PreAmp for SYBR® Green Assay: VAMP7, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: VAMP7, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0016352
Assay Design:		Intron-spanning
Chromosome Location:		X:155127859-155130217question
Amplicon Length:		82
Splice Variants Targeted:		ENST00000286448 ENST00000262640 ENST00000460621

Gene Information

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq Jun 2010]

Gene Symbol:		VAMP7
Gene Name:		vesicle-associated membrane protein 7
Aliases:		FLJ53045, FLJ53762, FLJ54296, SYBL1, TI-VAMP, TIVAMP, VAMP-7
RefSeq:		NC_000023.10 NC_000024.9 NT_167198.1 NT_167206.1 NG_021435.1
Ensembl:		ENSG00000124333
Entrez:		6845
Chromosome Mapping:		Xq28 and Yq12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	35.410000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.