PrimePCR™ SYBR® Green Assay: MYO1C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030723


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PrimePCR™ PreAmp for SYBR® Green Assay: MYO1C, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MYO1C, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0016642
Assay Design:		Intron-spanning
Chromosome Location:		17:1371738-1373472question
Amplicon Length:		110
Splice Variants Targeted:		ENST00000359786 ENST00000438665 ENST00000545534 ENST00000535856 ENST00000361007 ENST00000535421

Gene Information

This gene encodes a member of the unconventional myosin protein family which are actin-based molecular motors. The protein is found in the cytoplasm and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature but it is a distinct locus on chromosome 19. [provided by RefSeq Jul 2008]

Gene Symbol:		MYO1C
Gene Name:		myosin IC
Aliases:		FLJ23903, MMI-beta, MMIb, NMI, myr2
RefSeq:		NC_000017.10 NT_010718.16
Ensembl:		ENSG00000197879
Entrez:		4641
Chromosome Mapping:		17p13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	34.400000
Efficiency	106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.