PrimePCR™ SYBR® Green Assay: RAB8A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031005

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAB8A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAB8A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017075
Assay Design:   Intron-spanning
Chromosome Location:   19:16236295-16238307question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000300935

Gene Information

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97% 96% and 51% similarity with the dog RAB8 mouse MEL and mouse YPT1 proteins respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq Jul 2008]

Gene Symbol:   RAB8A
Gene Name:   RAB8A, member RAS oncogene family
Aliases:   MEL, RAB8
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000167461
Entrez:   4218
Chromosome Mapping:   19p13.1

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