PrimePCR™ SYBR® Green Assay: LIMK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033265

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LIMK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LIMK2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022037
Assay Design:   Intron-spanning
Chromosome Location:   22:31658703-31662010question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000406516 ENST00000331728 ENST00000333611 ENST00000340552 ENST00000436394

Gene Information

There are approximately 40 known eukaryotic LIM proteins so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK a downstream effector of Rho and the encoded protein in turn phosphorylates cofilin inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LIMK2
Gene Name:   LIM domain kinase 2
Aliases:   Not Available
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000182541
Entrez:   3985
Chromosome Mapping:   22q12.2

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