PrimePCR™ SYBR® Green Assay: HPGD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0040603


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PrimePCR™ PreAmp for SYBR® Green Assay: HPGD, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: HPGD, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0037880
Assay Design:		Intron-spanning
Chromosome Location:		4:175439134-175443202question
Amplicon Length:		173
Splice Variants Targeted:		ENST00000296522 ENST00000296521 ENST00000510901 ENST00000506910 ENST00000514584 ENST00000504433 ENST00000542498

Gene Information

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:		HPGD
Gene Name:		hydroxyprostaglandin dehydrogenase 15-(NAD)
Aliases:		15-PGDH, PGDH, PGDH1, SDR36C1
RefSeq:		NC_000004.11 NG_011689.1 NT_016354.19
Ensembl:		ENSG00000164120
Entrez:		3248
Chromosome Mapping:		4q34-q35

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.160000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.