PrimePCR™ SYBR® Green Assay: Ass1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0043623


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PrimePCR™ PreAmp for SYBR® Green Assay: Ass1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Ass1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0041407
Assay Design:		Intron-spanning
Chromosome Location:		2:31470278-31474760question
Amplicon Length:		86
Splice Variants Targeted:		ENSMUST00000078755 ENSMUST00000021761 ENSMUST00000071588 ENSMUST00000022544 ENSMUST00000176128 ENSMUST00000175744 ENSMUST00000176489 ENSMUST00000175905 ENSMUST00000067843 ENSMUST00000111569 ENSMUST00000072324 ENSMUST00000050658 ENSMUST00000063694 ENSMUST00000050535 ENSMUST00000107394 ENSMUST00000115044 ENSMUST00000023520 ENSMUST00000081713 ENSMUST00000006037 ENSMUST00000068304 ENSMUST00000071812 ENSMUST00000084117 ENSMUST00000030830 ENSMUST00000107151 ENSMUST00000021161 ENSMUST00000109690 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Ass1
Gene Name:		Argininosuccinate synthetase 1
Aliases:		AA408052, ASS, Ass-1, fold
RefSeq:		NC_000068.7 NT_039206.8
Ensembl:		ENSMUSG00000076441
Entrez:		11898
Chromosome Mapping:		2 B\|2 20.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	40.740000
Efficiency	83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.