PrimePCR™ SYBR® Green Assay: NDUFS8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040706

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NDUFS8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NDUFS8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037983
Assay Design:   exonic
Chromosome Location:   11:67800390-67800596question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000591806 ENST00000344663 ENST00000311401 ENST00000254661 ENST00000264122 ENST00000371518 ENST00000361387 ENST00000371471 ENST00000302342 ENST00000303868 ENST00000376396 ENST00000422185 ENST00000536628 ENST00000531085 ENST00000313468 ENST00000453471 ENST00000526339 ENST00000525419 ENST00000525628 ENST00000343110 ENST00000379717 ENST00000355467 ENST00000416005 ENST00000413371 ENST00000556601 ENST00000262424 ENST00000567845 ENST00000323851 ENST00000414097 ENST00000522476 ENST00000537882 ENST

Gene Information

This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase or Complex I a multimeric enzyme of the respiratory chain responsible for NADH oxidation ubiquinone reduction and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and as such is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq Mar 2010]

Gene Symbol:   NDUFS8
Gene Name:   NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
Aliases:   CI-23k, CI23KD, TYKY
RefSeq:   NC_000011.9 NG_007878.1 NG_012282.1 NG_017040.1 NT_167190.1
Ensembl:   ENSG00000110717
Entrez:   4728
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.600000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download