PrimePCR™ SYBR® Green Assay: TASP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049854

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TASP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TASP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042592
Assay Design:   exonic
Chromosome Location:   20:13610668-13610767question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000337743 ENST00000455532 ENST00000539805 ENST00000544472 ENST00000231706 ENST00000452508 ENST00000278616 ENST00000394126 ENST00000356271 ENST00000394128 ENST00000394127 ENST00000501516 ENST00000535071 ENST00000536318 ENST00000431269

Gene Information

This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL a protein required for the maintenance of HOX gene expression and TFIIA a basal transcription factor. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TASP1
Gene Name:   taspase, threonine aspartase, 1
Aliases:   C20orf13, FLJ20212, MGC39159, dJ585I14.2
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000089123
Entrez:   55617
Chromosome Mapping:   20p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download