PrimePCR™ SYBR® Green Assay: GINS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051627

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GINS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GINS2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044365
Assay Design:   exonic
Chromosome Location:   16:85721070-85721164question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000253462 ENST00000595355 ENST00000596233 ENST00000375749

Gene Information

The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611) Psf1 (GINS1; MIM 610608) Psf2 and Psf3 (GINS3; MIM 610610). The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al. 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM Mar 2008]

Gene Symbol:   GINS2
Gene Name:   GINS complex subunit 2 (Psf2 homolog)
Aliases:   HSPC037, PSF2, Pfs2
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000131153
Entrez:   51659
Chromosome Mapping:   16q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download