PrimePCR™ SYBR® Green Assay: USF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057521

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: USF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: USF2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056416
Assay Design:   exonic
Chromosome Location:   19:35761420-35761630question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000264661 ENST00000300258 ENST00000367866 ENST00000367865 ENST00000271411 ENST00000367862 ENST00000544745 ENST00000308736 ENST00000343550 ENST00000222305 ENST00000595068 ENST00000594064 ENST00000596380 ENST00000599471 ENST00000231721 ENST00000303400 ENST00000548825 ENST00000261897 ENST00000380281 ENST00000513602

Gene Information

This gene encodes a member of the basic helix-loop-helix leucine zipper family and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   USF2
Gene Name:   upstream transcription factor 2, c-fos interacting
Aliases:   FIP, bHLHb12
RefSeq:   NC_000019.9 NG_011563.1 NT_011109.16
Ensembl:   ENSG00000105698
Entrez:   7392
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.670000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download