PrimePCR™ Probe Assay: NFKBIL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NFKBIL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NFKBIL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040055
Assay Design:   exonic
Chromosome Location:   6:31526287-31526403question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000447098 ENST00000372192 ENST00000526629 ENST00000535109 ENST00000219251 ENST00000393919 ENST00000602320 ENST00000602850 ENST00000334370 ENST00000251268 ENST00000593647 ENST00000378073 ENST00000568225 ENST00000343304 ENST00000319080 ENST00000538698 ENST00000539039 ENST00000366272 ENST00000354852 ENST00000457787 ENST00000514226 ENST00000429814 ENST00000428467 ENST00000424652 ENST00000419623 ENST00000272433 ENST00000453377 ENST00000432160 ENST00000451911 ENST00000376146 ENST00000376148 ENST

Gene Information

This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   NFKBIL1
Gene Name:   nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1
Aliases:   IKBL, LST1, NFKBIL
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_012344.1
Ensembl:   ENSG00000204498
Entrez:   4795
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.150000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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