PrimePCR™ SYBR® Green Assay: CACNB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027073

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CACNB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CACNB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009163
Assay Design:   Intron-spanning
Chromosome Location:   10:18825067-18827152question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000324631 ENST00000352115 ENST00000377328 ENST00000282343 ENST00000377331 ENST00000396576 ENST00000377319 ENST00000377329 ENST00000377315

Gene Information

This gene encodes a subunit of a voltage-dependent calcium channel protein which is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome which is an autoimmune disorder. Mutations in this gene are associated with Brugada symdrome. Alternatively spliced variants have been identified for this gene. [provided by RefSeq Nov 2009]

Gene Symbol:   CACNB2
Gene Name:   calcium channel, voltage-dependent, beta 2 subunit
Aliases:   CACNLB2, CAVB2, FLJ23743, MYSB
RefSeq:   NC_000010.10 NT_008705.16 NG_016195.1
Ensembl:   ENSG00000165995
Entrez:   783
Chromosome Mapping:   10p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.170000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download