PrimePCR™ SYBR® Green Assay: PLA2G12A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028973

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLA2G12A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLA2G12A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013651
Assay Design:   Intron-spanning
Chromosome Location:   4:110635472-110638736question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000243501 ENST00000502283

Gene Information

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s such as PLA2G12A have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al. 2000 [PubMed 11031251]).[supplied by OMIM Mar 2008]

Gene Symbol:   PLA2G12A
Gene Name:   phospholipase A2, group XIIA
Aliases:   GXII, PLA2G12, ROSSY
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000123739
Entrez:   81579
Chromosome Mapping:   4q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.360000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download