PrimePCR™ SYBR® Green Assay: AD000671.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029600

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AD000671.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AD000671.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014888
Assay Design:   Intron-spanning
Chromosome Location:   19:36220127-36220973question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000222270 ENST00000420124

Gene Information

This gene encodes a protein which contains multiple domains including a CXXC zinc finger three PHD zinc fingers two FY-rich domains and a SET (suppressor of variegation enhancer of zeste and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene however the full length nature of the shorter transcript is not known. [provided by RefSeq Jul 2008]

Gene Symbol:   AD000671.1
Gene Name:   Histone-lysine N-methyltransferase MLL4
Aliases:   HRX2, KIAA0304, MLL2, TRX2, WBP7
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105663
Entrez:   9757
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.320000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download