PrimePCR™ SYBR® Green Assay: DDB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033098

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DDB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DDB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021586
Assay Design:   Intron-spanning
Chromosome Location:   11:61097476-61099052question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000301764 ENST00000539426 ENST00000535283 ENST00000542337 ENST00000543627

Gene Information

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq Jul 2008]

Gene Symbol:   DDB1
Gene Name:   damage-specific DNA binding protein 1, 127kDa
Aliases:   DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000167986
Entrez:   1642
Chromosome Mapping:   11q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download