PrimePCR™ Probe Assay: RPSA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPSA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPSA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041465
Assay Design:   Intron-spanning
Chromosome Location:   3:39449220-39450187question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000458337 ENST00000444166 ENST00000301821 ENST00000458478 ENST00000443003 ENST00000486528 ENST00000496398 ENST00000354585

Gene Information

Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity non-integrin family laminin receptor 1. This receptor has been variously called 67 kD laminin receptor 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist however most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RPSA
Gene Name:   ribosomal protein SA
Aliases:   37LRP, 67LR, LAMBR, LAMR1, LBP, LRP, SA, p40
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000168028
Entrez:   3921
Chromosome Mapping:   3p22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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