PrimePCR™ lncRNA SYBR® Green Assay:PCA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0182060. May amplify unspliced transcripts of an antisense gene.

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: PCA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: PCA3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104820
Assay Design:   exonic
Chromosome Location:   chr9:76786870-76786964question
Amplicon Length:   65
Refseq Transcripts Amplified:   NR_132312,NR_015342,NR_132313
Splice Variants Targeted:   ENST00000412654
LNCipedia Splice Variants Targeted:   PCA3:2 PCA3:1

Gene Information

Not Available

Gene Symbol:   PCA3
Gene Name:   prostate cancer associated 3 (non-protein coding) [Source:HGNC Symbol;Acc:HGNC:8637]
Aliases:   DD3, NCRNA00019, PCAT3
RefSeq:   Not Available
Ensembl:   ENSG00000225937
LNCipedia:   PCA3
Entrez:   50652
Chromosome Mapping:   9q21.2
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.950001
Efficiency 2.01

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download