PrimePCR™ Probe Assay: Tead4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Tead4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tead4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056564
Assay Design:   exonic
Chromosome Location:   6:128270966-128271056question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000004494 ENSMUST00000069799 ENSMUST00000130454 ENSMUST00000006311 ENSMUST00000112157 ENSMUST00000133118 ENSMUST00000143004 ENSMUST00000113122 ENSMUST00000113119 ENSMUST00000113121 ENSMUST00000113117 ENSMUST00000025292 ENSMUST00000036127 ENSMUST00000174837 ENSMUST00000173859 ENSMUST00000163734 ENSMUST00000022916 ENSMUST00000110305 ENSMUST00000020930 ENSMUST00000071093 ENSMUST00000106283 ENSMUST00000085558

Gene Information

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. This factor may play a role in the embryonic development of skeletal muscle. Alternatively spliced transcripts encoding distinct isoforms which are translated through the use of a non-AUG (AUU) initiation codon have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Tead4
Gene Name:   TEA domain family member 4
Aliases:   ETFR-2, Etfr2, FR-19, TEAD-4, TEF-3, Tcf13r1, Tef3, Tefr, Tefr1, Tefr1a
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000030353
Entrez:   21679
Chromosome Mapping:   6 F3|6 61.3 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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