PrimePCR™ SYBR® Green Assay: Fosl1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Fosl1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Fosl1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021046
Assay Design:		Intron-spanning
Chromosome Location:		19:5450239-5454448question
Amplicon Length:		119
Splice Variants Targeted:		ENSMUST00000044729 ENSMUST00000176824 ENSMUST00000103029 ENSMUST00000103028 ENSMUST00000055433 ENSMUST00000025850 ENSMUST00000006470 ENSMUST00000108154 ENSMUST00000131002 ENSMUST00000020732 ENSMUST00000047441 ENSMUST00000081658 ENSMUST00000052872 ENSMUST00000160249 ENSMUST00000162355 ENSMUST00000160649 ENSMUST00000178267 ENSMUST00000047746 ENSMUST00000139528 ENSMUST00000112584 ENSMUST00000112588 ENSMUST00000082177 ENSMUST00000050859 ENSMUST00000167746 ENSMUST00000110391 ENSMUST00000026236 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Fosl1
Gene Name:		Fos-like antigen 1
Aliases:		AW538199, Fra1, fra-1
RefSeq:		NC_000085.6 NT_082868.6
Ensembl:		ENSMUSG00000024912
Entrez:		14283
Chromosome Mapping:		19 A\|19 0.5 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.650000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.