PrimePCR™ SYBR® Green Assay: ARAF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049671

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARAF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARAF, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042409
Assay Design:   exonic
Chromosome Location:   X:47424407-47424666question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000377045 ENST00000377039 ENST00000290277

Gene Information

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jan 2012]

Gene Symbol:   ARAF
Gene Name:   v-raf murine sarcoma 3611 viral oncogene homolog
Aliases:   A-RAF, ARAF1, PKS2, RAFA1
RefSeq:   NC_000023.10 NG_008437.1 NG_016339.1 NT_079573.4
Ensembl:   ENSG00000078061
Entrez:   369
Chromosome Mapping:   Xp11.4-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download