PrimePCR™ SYBR® Green Assay: LHCGR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0052070


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PrimePCR™ PreAmp for SYBR® Green Assay: LHCGR, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: LHCGR, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044808
Assay Design:		exonic
Chromosome Location:		2:48914833-48914934question
Amplicon Length:		72
Splice Variants Targeted:		ENST00000294954 ENST00000405626 ENST00000403273 ENST00000401907 ENST00000344775

Gene Information

This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development including familial male precocious puberty also known as testotoxicosis hypogonadotropic hypogonadism Leydig cell adenoma with precocious puberty and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq Jul 2008]

Gene Symbol:		LHCGR
Gene Name:		luteinizing hormone/choriogonadotropin receptor
Aliases:		FLJ41504, HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5
RefSeq:		NC_000002.11 NG_008193.1 NT_022184.15
Ensembl:		ENSG00000138039
Entrez:		3973
Chromosome Mapping:		2p21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.870000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.