PrimePCR™ lncRNA SYBR® Green Assay:CDKN1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0180864

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: CDKN1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: CDKN1A, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103624
Assay Design:   exonic
Chromosome Location:   chr6:36686199-36686347question
Amplicon Length:   119
Refseq Transcripts Amplified:   NM_001220777,NM_078467,NM_001291549,NM_000389,NM_001220778
Splice Variants Targeted:   ENST00000405375 ENST00000448526 ENST00000615513 ENST00000244741
LNCipedia Splice Variants Targeted:   lnc-RAB44-3:1

Gene Information

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53 through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA) a DNA polymerase accessory factor and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases which thus leads to a dramatic activation of CDK2 and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   CDKN1A
Gene Name:   cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Aliases:   CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
RefSeq:   NC_000006.11 NG_009364.1 NT_007592.15
Ensembl:   ENSG00000124762
LNCipedia:   lnc-RAB44-3
Entrez:   1026
Chromosome Mapping:   6p21.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download