PrimePCR™ SYBR® Green Assay: S1pr1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: S1pr1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: S1pr1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020925
Assay Design:		Intron-spanning
Chromosome Location:		3:115712984-115714759question
Amplicon Length:		152
Splice Variants Targeted:		ENSMUST00000118835 ENSMUST00000145860 ENSMUST00000059115 ENSMUST00000055676

Gene Information

This gene encodes a G-protein-coupled receptor bound by the lysophospholipid sphingosine 1-phosphate. The gene product functions in endothelial cells and is involved in vascular and heart development. This receptor is highly expressed in T and B lymphocytes and it plays a role in T cell and B cell export from peripheral lymphoid organs. This protein is bound and downregulated by FTY720 an exogenous immunosuppressant drug studied in mouse disease models for multiple sclerosis in humans. [provided by RefSeq Jan 2010]

Gene Symbol:		S1pr1
Gene Name:		Sphingosine-1-phosphate receptor 1
Aliases:		AI849002, Edg1, Lpb1, S1p, S1p1
RefSeq:		NC_000069.6 NT_039240.8
Ensembl:		ENSMUSG00000045092
Entrez:		13609
Chromosome Mapping:		3 G1\|3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.620000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.