PrimePCR™ SYBR® Green Assay: CTBP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040910

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTBP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTBP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038188
Assay Design:   exonic
Chromosome Location:   10:126678049-126678216question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000391571 ENST00000431530 ENST00000332395 ENST00000215885 ENST00000402738 ENST00000407637 ENST00000264345 ENST00000337195 ENST00000309035 ENST00000334808 ENST00000531469 ENST00000494626 ENST00000411419 ENST00000527990 ENST00000254579 ENST00000303045 ENST00000435777 ENST00000245222 ENST00000601712 ENST00000600537 ENST00000340932 ENST00000392161

Gene Information

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. [provided by RefSeq Jul 2008]

Gene Symbol:   CTBP2
Gene Name:   C-terminal binding protein 2
Aliases:   Not Available
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000175029
Entrez:   1488
Chromosome Mapping:   10q26.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.560000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download