PrimePCR™ SYBR® Green Assay: MAPK3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027795

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAPK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAPK3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010939
Assay Design:   Intron-spanning
Chromosome Location:   16:30129834-30133298question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000263025 ENST00000484663 ENST00000395200 ENST00000395202 ENST00000478356 ENST00000395199 ENST00000495629 ENST00000481230 ENST00000322266 ENST00000403394

Gene Information

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act in a signaling cascade that regulates various cellular processes such as proliferation differentiation and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPK3
Gene Name:   mitogen-activated protein kinase 3
Aliases:   ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000102882
Entrez:   5595
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download