PrimePCR™ SYBR® Green Assay: CTNNB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053779

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTNNB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTNNB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046518
Assay Design:   exonic
Chromosome Location:   3:41266084-41266226question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000426215 ENST00000405570 ENST00000431914 ENST00000396183 ENST00000349496 ENST00000453024 ENST00000396185 ENST00000450969 ENST00000441708

Gene Information

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally this protein binds to the product of the APC gene which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC) pilomatrixoma (PTR) medulloblastoma (MDB) and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   CTNNB1
Gene Name:   catenin (cadherin-associated protein), beta 1, 88kDa
Aliases:   CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
RefSeq:   NC_000003.11 NT_022517.18 NG_013302.1
Ensembl:   ENSG00000168036
Entrez:   1499
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download