PrimePCR™ SYBR® Green Assay: PTPN6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: PTPN6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTPN6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005383
Assay Design:   Exonic
Chromosome Location:   12:7069977-7070080question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000399448 ENST00000318974 ENST00000447931 ENST00000456013

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains which act as protein phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene which encode distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPN6
Gene Name:   protein tyrosine phosphatase, non-receptor type 6
Aliases:   HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000111679
Entrez:   5777
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download