PrimePCR™ SYBR® Green Assay: CTSD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTSD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTSD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005697
Assay Design:   Exonic
Chromosome Location:   11:1774090-1774221question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000236671

Gene Information

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains both produced from a single protein precursor. This proteinase which is a member of the peptidase C1 family has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases including breast cancer and possibly Alzheimer disease. [provided by RefSeq Jul 2008]

Gene Symbol:   CTSD
Gene Name:   cathepsin D
Aliases:   CLN10, CPSD, MGC2311
RefSeq:   NC_000011.9 NG_008655.1 NT_009237.18
Ensembl:   ENSG00000117984
Entrez:   1509
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.970000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download