PrimePCR™ SYBR® Green Assay: F3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039176

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: F3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: F3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036452
Assay Design:   exonic
Chromosome Location:   1:94995818-94995939question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000334047 ENST00000370207

Gene Information

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades which circulate as nonfunctional precursors this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular transmembrane and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   F3
Gene Name:   coagulation factor III (thromboplastin, tissue factor)
Aliases:   CD142, FLJ17960, TF, TFA
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117525
Entrez:   2152
Chromosome Mapping:   1p22-p21

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