PrimePCR™ SYBR® Green Assay: H3F3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040838

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: H3F3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: H3F3A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038115
Assay Design:   exonic
Chromosome Location:   1:226253368-226253467question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000446824 ENST00000366816 ENST00000366815 ENST00000366814 ENST00000366813

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq Jul 2008]

Gene Symbol:   H3F3A
Gene Name:   H3 histone, family 3A
Aliases:   H3.3A, H3F3, H3F3B, MGC87782, MGC87783
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000163041
Entrez:   3020
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download