PrimePCR™ SYBR® Green Assay: VPS45, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0051965


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PrimePCR™ PreAmp for SYBR® Green Assay: VPS45, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: VPS45, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044703
Assay Design:		exonic
Chromosome Location:		1:150054840-150064041question
Amplicon Length:		109
Splice Variants Targeted:		ENST00000373677 ENST00000510661 ENST00000540800 ENST00000535692 ENST00000369130 ENST00000419023 ENST00000535106 ENST00000369128 ENST00000344517 ENST00000579153

Gene Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family and shows a high degree of sequence similarity to mouse rat and yeast Vps45. The exact function of this gene is not known but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins including inflammatory mediators. [provided by RefSeq Jul 2008]

Gene Symbol:		VPS45
Gene Name:		vacuolar protein sorting 45 homolog (S. cerevisiae)
Aliases:		H1, H1VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
RefSeq:		NC_000001.10 NT_004487.19
Ensembl:		ENSG00000136631
Entrez:		11311
Chromosome Mapping:		1q21.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	36.550000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.