PrimePCR™ SYBR® Green Assay: ADCY9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053227


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PrimePCR™ PreAmp for SYBR® Green Assay: ADCY9, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ADCY9, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045966
Assay Design:		exonic
Chromosome Location:		16:4163904-4164023question
Amplicon Length:		90
Splice Variants Targeted:		ENST00000294016 ENST00000572288

Gene Information

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors protein kinases and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin calcium and somatostatin. [provided by RefSeq Jul 2008]

Gene Symbol:		ADCY9
Gene Name:		adenylate cyclase 9
Aliases:		AC9, FLJ12046
RefSeq:		NC_000016.9 NG_011434.1 NT_010393.16
Ensembl:		ENSG00000162104
Entrez:		115
Chromosome Mapping:		16p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.940000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.