PrimePCR™ SYBR® Green Assay: MAP4K2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053786

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAP4K2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAP4K2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046525
Assay Design:   exonic
Chromosome Location:   11:64556610-64556734question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000377350 ENST00000294066

Gene Information

The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues its expression in lymphoid follicles is restricted to the cells of germinal centre where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2) which is involved in the activation of MAP3K1/MEKK1. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP4K2
Gene Name:   mitogen-activated protein kinase kinase kinase kinase 2
Aliases:   BL44, GCK, RAB8IP
RefSeq:   NC_000011.9 NG_008929.1 NT_167190.1
Ensembl:   ENSG00000168067
Entrez:   5871
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.620000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download