PrimePCR™ SYBR® Green Assay: DST, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027570

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DST, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010403
Assay Design:   Intron-spanning
Chromosome Location:   6:56505285-56506767question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000244364 ENST00000370788 ENST00000361203 ENST00000439203 ENST00000520645 ENST00000370765 ENST00000518935 ENST00000449297 ENST00000370754 ENST00000370769 ENST00000421834 ENST00000446842 ENST00000312431

Gene Information

This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue anchoring neural intermediate filaments to the actin cytoskeleton and some isoforms are expressed in epithelial tissue anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq Mar 2010]

Gene Symbol:   DST
Gene Name:   dystonin
Aliases:   BP240, BPA, BPAG1, CATX-15, CATX15, D6S1101, DKFZp564B2416, DMH, DT, FLJ46791, KIAA0465, KIAA1470, MACF2
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000151914
Entrez:   667
Chromosome Mapping:   6p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 34.360000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download