PrimePCR™ lncRNA SYBR® Green Assay:DUSP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181175

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DUSP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DUSP4, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103935
Assay Design:   exonic
Chromosome Location:   chr8:29334881-29335003question
Amplicon Length:   93
Refseq Transcripts Amplified:   NM_001394,NM_057158,XM_011544428
Splice Variants Targeted:   ENST00000240100
LNCipedia Splice Variants Targeted:   lnc-DUSP4-6:1

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1 ERK2 and JNK is expressed in a variety of tissues and is localized in the nucleus. Two alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. In addition multiple polyadenylation sites have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP4
Gene Name:   dual specificity phosphatase 4
Aliases:   HVH2, MKP-2, MKP2, TYP
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000120875
LNCipedia:   lnc-DUSP4-6
Entrez:   1846
Chromosome Mapping:   8p12-p11
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download