PrimePCR™ SYBR® Green Assay: Ppp1ca, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0057959

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Ppp1ca, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ppp1ca, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049533
Assay Design:   exonic
Chromosome Location:   19:4193024-4193163question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000023884 ENSMUST00000170374 ENSMUST00000005829 ENSMUST00000076316 ENSMUST00000058682 ENSMUST00000046094 ENSMUST00000108393 ENSMUST00000017832 ENSMUST00000004681 ENSMUST00000111070 ENSMUST00000042646 ENSMUST00000026750 ENSMUST00000112513 ENSMUST00000021177 ENSMUST00000103026 ENSMUST00000090433 ENSMUST00000180575

Gene Information

Description Not Available

Gene Symbol:   Ppp1ca
Gene Name:   Protein phosphatase 1, catalytic subunit, alpha isoform
Aliases:   Ppp1c, dism2
RefSeq:   NC_000085.6 NT_082868.6 NW_004058054.1
Ensembl:   ENSMUSG00000040385
Entrez:   19045
Chromosome Mapping:   19|19 E3-F2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download