PrimePCR™ SYBR® Green Assay: Fgfr2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Fgfr2, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Fgfr2, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021280
Assay Design:		Intron-spanning
Chromosome Location:		7:130177834-130180460question
Amplicon Length:		98
Splice Variants Targeted:		ENSMUST00000117872 ENSMUST00000122054 ENSMUST00000120187 ENSMUST00000121064 ENSMUST00000117754 ENSMUST00000120715 ENSMUST00000119260 ENSMUST00000117089 ENSMUST00000117691 ENSMUST00000117858 ENSMUST00000120141 ENSMUST00000117357 ENSMUST00000122448 ENSMUST00000118296 ENSMUST00000121080 ENSMUST00000117073

Gene Information

Description Not Available

Gene Symbol:		Fgfr2
Gene Name:		Fibroblast growth factor receptor 2
Aliases:		AU043015, AW556123, Bek, Fgfr-2, Fgfr-7, Fgfr7, KGFR, KGFRTr, svs
RefSeq:		NC_000073.6 NT_039433.8
Ensembl:		ENSMUSG00000030849
Entrez:		14183
Chromosome Mapping:		7 F3\|7

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.330000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.