PrimePCR™ SYBR® Green Assay: JAR1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: JAR1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: JAR1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052196
Assay Design:   Exonic
Chromosome Location:   2:19036058-19036207question
Amplicon Length:   120
Splice Variants Targeted:   AT5G06120.2 AT5G06120.3 AT5G06120.4 AT2G46370.1 AT2G46370.2 AT2G46370.3 AT2G46370.4

Gene Information

Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.

Gene Symbol:   JAR1
Gene Name:   Auxin-responsive GH3 family protein
Aliases:   Not Available
RefSeq:   NM_001202828 NM_180122 NM_130200 NM_001084600
Ensembl:   AT2G46370
Entrez:   819244
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download