PrimePCR™ Probe Assay: P5CS1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: P5CS1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: P5CS1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024890
Assay Design:   Exonic
Chromosome Location:   2:16599224-16599318question
Amplicon Length:   65
Splice Variants Targeted:   AT4G30520.1 AT2G39800.1 AT2G39800.2 AT2G39800.3 AT2G39800.4

Gene Information

encodes a delta1-pyrroline-5-carboxylate synthase that catalyzes the rate-limiting enzyme in the biosynthesis of proline. Gene is expressed in reproductive organs and tissues under non-stress conditions but in the whole plant under water-limiting condition. Expression is also induced by abscisic acid and salt stress in a light-dependent manner. P5CS1 appears to be involved in salt stress responses related to proline accumulation, including protection from reactive oxidative species. P5CS1 appears to be present in different cells and/or different subcellular locations from P5CS2 in a tissue-dependent manner.

Gene Symbol:   P5CS1
Gene Name:   Delta1-pyrroline-5-carboxylate synthase 1
Aliases:   Not Available
RefSeq:   NM_001202786 NM_001202785 NM_129539 NM_201912
Ensembl:   AT2G39800
Entrez:   818566
Chromosome Mapping:   Not Available

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