PrimePCR™ Probe Assay: PPDK, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPDK, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPDK, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026316
Assay Design:   Exonic
Chromosome Location:   4:8865477-8865667question
Amplicon Length:   161
Splice Variants Targeted:   AT4G15530.1 AT4G15530.2 AT4G15530.3 AT4G15530.4 AT4G15530.5 AT4G15530.6 AT2G33240.1

Gene Information

Encodes a dual-targeted protein believed to act as a pyruvate, orthophosphate dikinase. These enzymes are normally associated with C4 photosynthesis which does not occur in Arabidopsis. However, PPDK may play a role in remobilizing nitrogen during leaf senescence in Arabidopsis. The product of the long transcript (.1 gene model) was shown to be targeted to the chloroplast, whereas the shorter transcript (no targeting sequence) accumulates in the cytosol. The two proteins were also found to be expressed in slightly different tissues.

Gene Symbol:   PPDK
Gene Name:   Pyruvate orthophosphate dikinase
Aliases:   Not Available
RefSeq:   NM_179060 NM_001084926 NM_001036570 NM_001125515 NM_001084927 NM_117643
Ensembl:   AT4G15530
Entrez:   827226
Chromosome Mapping:   Not Available

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