PrimePCR™ SYBR® Green Assay: CYB561D1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0023998


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PrimePCR™ PreAmp for SYBR® Green Assay: CYB561D1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CYB561D1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0001251
Assay Design:		Exonic
Chromosome Location:		1:110038900-110039050question
Amplicon Length:		121
Splice Variants Targeted:		ENST00000496961 ENST00000533024 ENST00000310611 ENST00000527072 ENST00000420578 ENST00000369868 ENST00000430195

Gene Information

Description Not Available

Gene Symbol:		CYB561D1
Gene Name:		cytochrome b-561 domain containing 1
Aliases:		FLJ39035, FLJ44753, MGC138204
RefSeq:		NC_000001.10 NT_032977.9
Ensembl:		ENSG00000174151
Entrez:		284613
Chromosome Mapping:		1p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.040000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.